restoretm plus western blot stripping buffer Search Results


tmb one component substrate bethyl  (Bethyl)
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Bethyl tmb one component substrate bethyl
Tmb One Component Substrate Bethyl, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restoretm western blot stripping buffer  (Thermo Fisher)
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Thermo Fisher restoretm western blot stripping buffer
Restoretm Western Blot Stripping Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blot stripping buffer restoretm  (Thermo Fisher)
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Thermo Fisher blot stripping buffer restoretm
Blot Stripping Buffer Restoretm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stripping buffer  (Thermo Fisher)
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Thermo Fisher stripping buffer
Stripping Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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restoretm western blot stripping buffer  (EuroClone)
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EuroClone restoretm western blot stripping buffer
Restoretm Western Blot Stripping Buffer, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intelligent dark box las-3000  (FUJIFILM)
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Intelligent Dark Box Las 3000, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ponceau s  (Merck & Co)
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Merck & Co ponceau s
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rabbit polyclonal anti trpm4 antibody antigenic peptide  (Aviva Systems)
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Aviva Systems rabbit polyclonal anti trpm4 antibody antigenic peptide
Rabbit Polyclonal Anti Trpm4 Antibody Antigenic Peptide, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igg monoclonal antibody stat3  (Becton Dickinson)
90
Becton Dickinson mouse igg monoclonal antibody stat3
Rats were subjected to sham protocol (Sham, n = 3), unresuscitated T/HS (UHS, n = 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n = 4), T/HS treated with IL-6 at the beginning of resuscitation (SBR50/IL-6, n = 4), T/HS preceded by treatment with GQ-oligodeoxynucleotide (GQ-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6/G, n = 3), or T/HS preceded by treatment with nonspecific-ODN (NS-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6N, n = 3). The livers were harvested 60 minutes after the start of resuscitation. MPO staining was performed in paraformaldehyde-fixed livers. Representative photomicrographs of 1000x fields of liver specimens from each experimental group are shown (2A). Arrows indicate MPO-positive cells. In , MPO-positive cells were counted in 20 hpf; data presented are the means ± SEM of each group (n≥5). Bars marked with an asterisk (*) differ significantly within the pair (p<0.05). shows representative immunoblots of protein extracts of whole liver from each group, a negative control (NC) cells and positive control (PC) cells developed with monoclonal antibodies to either phosphorylated <t>(p)Stat3</t> and total (t)Stat3. Bands representing Stat3α, Stat3β, and Stat3δ isoforms are indicated on the right. Heavy lines separate representative samples from individual gels. shows the mean densitometric ratio of (p)Stat3 to (t)Stat3 bands ± standard deviation of the groups (n≥3 for each). Statistically significant (p<0.05) intergroup comparisons are indicated by “*”, “†”, and “§”, respectively.
Mouse Igg Monoclonal Antibody Stat3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse igg monoclonal antibody stat3 - by Bioz Stars, 2026-08
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p53  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology p53
Rats were subjected to sham protocol (Sham, n = 3), unresuscitated T/HS (UHS, n = 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n = 4), T/HS treated with IL-6 at the beginning of resuscitation (SBR50/IL-6, n = 4), T/HS preceded by treatment with GQ-oligodeoxynucleotide (GQ-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6/G, n = 3), or T/HS preceded by treatment with nonspecific-ODN (NS-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6N, n = 3). The livers were harvested 60 minutes after the start of resuscitation. MPO staining was performed in paraformaldehyde-fixed livers. Representative photomicrographs of 1000x fields of liver specimens from each experimental group are shown (2A). Arrows indicate MPO-positive cells. In , MPO-positive cells were counted in 20 hpf; data presented are the means ± SEM of each group (n≥5). Bars marked with an asterisk (*) differ significantly within the pair (p<0.05). shows representative immunoblots of protein extracts of whole liver from each group, a negative control (NC) cells and positive control (PC) cells developed with monoclonal antibodies to either phosphorylated <t>(p)Stat3</t> and total (t)Stat3. Bands representing Stat3α, Stat3β, and Stat3δ isoforms are indicated on the right. Heavy lines separate representative samples from individual gels. shows the mean densitometric ratio of (p)Stat3 to (t)Stat3 bands ± standard deviation of the groups (n≥3 for each). Statistically significant (p<0.05) intergroup comparisons are indicated by “*”, “†”, and “§”, respectively.
P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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recombinant human furin protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human furin protein, cf
Rats were subjected to sham protocol (Sham, n = 3), unresuscitated T/HS (UHS, n = 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n = 4), T/HS treated with IL-6 at the beginning of resuscitation (SBR50/IL-6, n = 4), T/HS preceded by treatment with GQ-oligodeoxynucleotide (GQ-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6/G, n = 3), or T/HS preceded by treatment with nonspecific-ODN (NS-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6N, n = 3). The livers were harvested 60 minutes after the start of resuscitation. MPO staining was performed in paraformaldehyde-fixed livers. Representative photomicrographs of 1000x fields of liver specimens from each experimental group are shown (2A). Arrows indicate MPO-positive cells. In , MPO-positive cells were counted in 20 hpf; data presented are the means ± SEM of each group (n≥5). Bars marked with an asterisk (*) differ significantly within the pair (p<0.05). shows representative immunoblots of protein extracts of whole liver from each group, a negative control (NC) cells and positive control (PC) cells developed with monoclonal antibodies to either phosphorylated <t>(p)Stat3</t> and total (t)Stat3. Bands representing Stat3α, Stat3β, and Stat3δ isoforms are indicated on the right. Heavy lines separate representative samples from individual gels. shows the mean densitometric ratio of (p)Stat3 to (t)Stat3 bands ± standard deviation of the groups (n≥3 for each). Statistically significant (p<0.05) intergroup comparisons are indicated by “*”, “†”, and “§”, respectively.
Recombinant Human Furin Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Rats were subjected to sham protocol (Sham, n = 3), unresuscitated T/HS (UHS, n = 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n = 4), T/HS treated with IL-6 at the beginning of resuscitation (SBR50/IL-6, n = 4), T/HS preceded by treatment with GQ-oligodeoxynucleotide (GQ-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6/G, n = 3), or T/HS preceded by treatment with nonspecific-ODN (NS-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6N, n = 3). The livers were harvested 60 minutes after the start of resuscitation. MPO staining was performed in paraformaldehyde-fixed livers. Representative photomicrographs of 1000x fields of liver specimens from each experimental group are shown (2A). Arrows indicate MPO-positive cells. In , MPO-positive cells were counted in 20 hpf; data presented are the means ± SEM of each group (n≥5). Bars marked with an asterisk (*) differ significantly within the pair (p<0.05). shows representative immunoblots of protein extracts of whole liver from each group, a negative control (NC) cells and positive control (PC) cells developed with monoclonal antibodies to either phosphorylated (p)Stat3 and total (t)Stat3. Bands representing Stat3α, Stat3β, and Stat3δ isoforms are indicated on the right. Heavy lines separate representative samples from individual gels. shows the mean densitometric ratio of (p)Stat3 to (t)Stat3 bands ± standard deviation of the groups (n≥3 for each). Statistically significant (p<0.05) intergroup comparisons are indicated by “*”, “†”, and “§”, respectively.

Journal: PLoS ONE

Article Title: IL-6-Mediated Activation of Stat3α Prevents Trauma/Hemorrhagic Shock-Induced Liver Inflammation

doi: 10.1371/journal.pone.0021449

Figure Lengend Snippet: Rats were subjected to sham protocol (Sham, n = 3), unresuscitated T/HS (UHS, n = 3), T/HS treated with placebo at the beginning of resuscitation (SBR50, n = 4), T/HS treated with IL-6 at the beginning of resuscitation (SBR50/IL-6, n = 4), T/HS preceded by treatment with GQ-oligodeoxynucleotide (GQ-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6/G, n = 3), or T/HS preceded by treatment with nonspecific-ODN (NS-ODN) 24 hours prior to resuscitation with IL-6 (SBR50/IL-6N, n = 3). The livers were harvested 60 minutes after the start of resuscitation. MPO staining was performed in paraformaldehyde-fixed livers. Representative photomicrographs of 1000x fields of liver specimens from each experimental group are shown (2A). Arrows indicate MPO-positive cells. In , MPO-positive cells were counted in 20 hpf; data presented are the means ± SEM of each group (n≥5). Bars marked with an asterisk (*) differ significantly within the pair (p<0.05). shows representative immunoblots of protein extracts of whole liver from each group, a negative control (NC) cells and positive control (PC) cells developed with monoclonal antibodies to either phosphorylated (p)Stat3 and total (t)Stat3. Bands representing Stat3α, Stat3β, and Stat3δ isoforms are indicated on the right. Heavy lines separate representative samples from individual gels. shows the mean densitometric ratio of (p)Stat3 to (t)Stat3 bands ± standard deviation of the groups (n≥3 for each). Statistically significant (p<0.05) intergroup comparisons are indicated by “*”, “†”, and “§”, respectively.

Article Snippet: The membrane was then stripped (using RestoreTM Western Blot Stripping Buffer, PIERCE, Rockford, IL) and immuoblotting performed to detect total Stat3 protein using mouse IgG monoclonal antibody to Stat3 (BD Biosciences, Rockville, MD; 1∶1000 dilution) as described before , , .

Techniques: Staining, Western Blot, Negative Control, Positive Control, Standard Deviation